Reporter and adventurer Pintin Le Pew (Pepé Le Pew), along with his apprentice Fifi La Fume and his dog Charlie join forces with his new friend Penelope Pussycat, the loudmouthed Captain Foghorn Legdock (Foghorn Leghorn) and the detectives Babbitson and Catstellson (Babbit and Catstello) to save Professor Calcul E. Coyote (Wile E. Coyote) from the evil Yosemipopoulos Sam (Yosemite Sam). On the way, they also met other characters like Prissy Castafiore (Miss Prissy) and Jolyon Dawg (Barnyard Dawg).
- Pepé Le Pew (as Pintin Le Pew, voiced by Maurice LaMarche)
- Fifi La Fume (voiced by Kath Soucie)
- Charlie Dog (voiced by Frank Welker)
- Penelope Pussycat (voiced by Tress MacNeille)
- Foghorn Leghorn (as Captain Foghorn Legdock, voiced by Jeff Bennett)
- Babbit and Catstello (as Babbitson and Catstellson, voiced by Billy West and Jim Cummings, respectively)
- Wile E. Coyote (as Professor Calcul E. Coyote, voiced by Maurice LaMarche)
- Yosemite Sam (as Yosemipopoulos Sam, voiced by Maurice LaMarche)
- Miss Prissy (as Prissy Castafiore, voiced by Tress MacNeille)
- Barnyard Dawg (as Jolyon Dawg, voiced by Maurice LaMarche)
- Bugs Bunny (non-speaking cameo)
- Daffy Duck (non-speaking cameo)
- This segment serves as a spoof to The Adventures of Tintin.