Dot gets tired from her brothers' antics and decides to leave the trio, leading to Yakko and Wakko trying to find a substitute. They eventually find a beautiful girl and fall in love with her, but soon start to miss their younger sister. Meanwhile, Dot tries to get used to her life without her brothers, but she begins to miss them too, so she tries to come back, which it means get rid of her replacement.
- Yakko, Wakko and Dot Warner (voiced by Rob Paulsen, Jess Harnell and Tress MacNeille, respectively)
- Replacement Sister (voiced by Tara Strong)
- Wile E. Coyote (cameo, voiced by Maurice LaMarche)
- "(angrily) I'm so sick of you two, I'm gonna quit" - Dot Warner.
- "(entering the room) Are the auditions open?" - Replacement Sister.
- "(falling in love with her, whistling) Hello, nurse!" - Yakko and Wakko Warner.
- "(when Dot falls down from a canyon) Hey! Get out of the way for someone who is more experienced in falling!" - Wile E. Coyote.
- "A question: now that Dot's back, what we could do with her?" - Yakko Warner.
- "I think I have an idea..." - Dot Warner.